5 dihydrotestosterone dht  (Melone Pharmaceutical Co Ltd)

Journal: Biomedicines

Article Title: An Ovarian Steroid Metabolomic Pathway Analysis in Basal and Polycystic Ovary Syndrome (PCOS)-like Gonadotropin Conditions Reveals a Hyperandrogenic Phenotype Measured by Mass Spectrometry

doi: 10.3390/biomedicines10071646

Figure Lengend Snippet: Ovarian explant steroid hormone production across 28-day culture differs in response to different pituitary hormone concentrations. All 7 hormones (estrone, estradiol, progesterone, testosterone, androstenedione, dihydrotestosterone (DHT), and dehydroepiandrosterone (DHEA)) over 28 days for ovaries in ( A ) G0, standard media, ( B ) G10, 1:1 physiologic ratio media, and ( C ) G30, 3:1 PCOS-like ratio media, respectively. ( D ) Estrogens (estrone + estradiol), ( E ) progesterone, and ( F ) androgens (testosterone + androstenedione + DHT + DHEA) across 28 days of culture. Data are presented as mean ± SEM. n = 11–12 samples/group.

Article Snippet: Estrone (E1, E-075), 17-β-estradiol (E2, E-060), progesterone (P, P-069), testosterone (T, T-037), androstenedione (A, A-075), 5-α-dihydrotestosterone (DHT, D-073), and dehydroepiandrosterone (DHEA, D-063) purchased from Sigma-Aldrich (as Cerilliant brand) were used to fabricate hormone calibration standards.

Techniques:

Journal: Biomedicines

Article Title: An Ovarian Steroid Metabolomic Pathway Analysis in Basal and Polycystic Ovary Syndrome (PCOS)-like Gonadotropin Conditions Reveals a Hyperandrogenic Phenotype Measured by Mass Spectrometry

doi: 10.3390/biomedicines10071646

Figure Lengend Snippet: Cumulative hormones production reveals excess testosterone and DHT production in G30 group. Cumulative production of each steroid hormone was calculated by taking the sum of hormone productions from samples collected on alternate days from culture day 2–culture day 27. Cumulative production for ( A ) estrone, ( B ) estradiol, ( C ) progesterone, ( D ) testosterone, ( E ) androstenedione, ( F ) DHEA (dehydroepiandrosterone) and ( G ) DHT (dihydrotestosterone). Data are presented as the mean ± SEM. n = 11–12 samples/group. Statistical significance determined with one-way ANOVA with Tukey’s multiple comparison test and/or post hoc test for linear trend (results of the latter test cannot be illustrated on the graphs above, but corresponding p values are included in the text of the Results section). * signifies p < 0.05, and *** signifies p < 0.001. G0 = standard media (no hCG), G10 = physiologic ratio media (1:1 hCG:FSH), G30 = PCOS-like ratio (3:1 hCG:FSH). Abbreviations: hCG = human chorionic gonadotropin, FSH = follicle stimulating hormone.

Article Snippet: Estrone (E1, E-075), 17-β-estradiol (E2, E-060), progesterone (P, P-069), testosterone (T, T-037), androstenedione (A, A-075), 5-α-dihydrotestosterone (DHT, D-073), and dehydroepiandrosterone (DHEA, D-063) purchased from Sigma-Aldrich (as Cerilliant brand) were used to fabricate hormone calibration standards.

Techniques: